Atlas of Cellular Dynamics during Zebrafish Adult Kidney Regeneration

Figure 2

Histological staining reveals the process of regeneration after gentamicin-induced injury in adult zebrafish. (a) H&E and (b) PAS staining demonstrated extensive nephron damage and tubule destruction, followed by regenerative events that were completed by 21 dpi. The formation of basophilic cellular aggregates (darkest purple cellular staining) was indicative of new nephron formation. Both time courses were completed over a three-week period. 60x. DT: distal tubule; G: glomerulus; PT: proximal tubule. Yellow labels: asterisk () indicates luminal debris; double asterisk () indicates restoration of cells to tubules; arrowhead indicates neonephrogenic cluster; arrow indicates neonephron with visible lumen.

(a)

(b)