Atlas of Cellular Dynamics during Zebrafish Adult Kidney Regeneration

Figure 3

Dynamics of slc20a1a expression over the course of zebrafish adult kidney injury and regeneration. (a) Whole mount in situ hybridization of adult kidneys over a two-week injury time course revealed the initial absence of slc20a1a transcripts, followed by an increase in transcript levels and domains to near-normal levels by 14 dpi. Throughout the time course, new nephrons first appeared as small cellular aggregates that form coils and eventually elongated into PCT segments that are indistinguishable from preexisting, regenerated PCT tubules. 3x magnification. (b) Representative images of the progressive stages of nephron formation. Scale bar, 25 μm. (c) Quantification shows that slc20a1a positive structures during regeneration. Labels: indicates ; indicates ; black indicates aggregate; green indicates coil; purple indicates segment.

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