Atlas of Cellular Dynamics during Zebrafish Adult Kidney Regeneration

Figure 4

Detection of proximal tubule cell death after gentamicin injury. (a) Kidney tissue was assayed for TUNEL-positive cells in uninjured kidneys and at 1, 3, 5, 7, and 10 dpi. The peak of TUNEL reactivity in cells was identified within LTL-positive proximal tubules at 1, 3, and 5 days after kidney injury. By 7 and 10 days, the level of TUNEL-positive cells decreased, returning to basal levels previously established in untreated kidneys. Proximal tubules were labeled by LTL. (b) Quantification of TUNEL labeling in nephron proximal segments. Scale bar, 25 μm. Labels: indicates ; indicates .

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