Atlas of Cellular Dynamics during Zebrafish Adult Kidney Regeneration

Figure 5

Cell proliferation in the regenerating proximal tubule epithelium and in neonephrogenic clusters. Kidney tissue was assayed for PCNA-positive nuclei (red), and proximal tubules were identified by LTL labeling (green). Nuclei were stained with DAPI (blue). (a) Most epithelial proliferation occurred at 3, 5, and 7 days after kidney injury. Scale bar, 25 μm. (b) Quantification of PCNA expression in nephron proximal tubular segments. Labels: indicates ; indicates . (c) Epithelial proliferation marked by PCNA (red) occurred at high levels in cellular aggregates at 3 and 5 dpi. At 7 dpi, aggregates that have formed lumens continued to express intense levels of PCNA. At 10 dpi, PCNA was still abundant in tubules that have become LTL-positive (green).

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