Atlas of Cellular Dynamics during Zebrafish Adult Kidney Regeneration

Figure 7

Changes in ELF-97 staining and proximal tubule functionality after gentamicin injury. Whole mount labeling of (a) alkaline phosphatase reactivity and (b) uptake of fluoro-ruby dextran in the zebrafish kidney during recovery from AKI. (a) Untreated kidneys contained discrete PCT-PST structures (white box) that were destroyed at 1 dpi (white arrowheads). Restoration of discrete PCT-PST structures was completed by 14 dpi. (b) Nephron reabsorption was visualized in injured kidneys, in which the PCT was demarcated by uptake of fluoro-ruby dextran, while at 1 dpi, such structures were absent (white arrowheads). Partial uptake was sporadically observed at 5–7 dpi but was not consistent throughout the kidney organ until 21 dpi when PCT segments were distinctly visualized (white box).

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