Atlas of Cellular Dynamics during Zebrafish Adult Kidney Regeneration

Figure 8

The Pax2 transcription factor marks cells in the regenerating proximal tubule and neonephrogenic clusters. (a) Expression of Pax2 in the regenerating kidney tubule. Pax2-positive nuclei (red) were identified in kidney tubule cryosections at baseline and over a two-week span after injury. Elevated Pax2 expression was detected at 5, 7, and 10 dpi. Nuclei were stained with DAPI (blue). Scale bar, 25 μm. (b) Intense Pax2 immunoreactivity demarcated neonephrogenic units. High levels of Pax2 expression (red) were present in coil-like structures at 5 dpi. At 10 dpi, Pax2 continued to stain profound numbers of cells in the coil-like neonephron structures. Nuclei are stained with DAPI (blue). Scale bar, 25 μm.

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