Morphometric Analysis of Human Embryonic Stem Cell-Derived Ventricular Cardiomyocytes: Determining the Maturation State of a Population by Quantifying Parameters in Individual Cells

Figure 1

Effect of isoproterenol and oleic acid on the shape of hESC-vCMs. ((a) panel (A)) Schematic illustration to show that circularity values of 0 and 1 represent a straight line and perfect circle, respectively. ((a) panel (B), (a) panel (C)) Representative examples of elongated and rounded hESC-vCMs (labeled with an α-actinin antibody) that have circularity values of ((a) panel (B)) 0.31 AU and ((a) panel (C)) 0.97 AU, respectively. Scale bar is 30 μm. (b–g) Histograms to show the distribution of cells (in %) exhibiting a particular circularity value in (b) untreated controls and (c–g) following treatment with (c) 0.3 μM isoproterenol, (d) 100 μM oleic acid, (e) 200 μM oleic acid, (f) 0.3 μM isoproterenol plus 100 μM oleic acid, or (g) 0.3 μM isoproterenol plus 200 μM oleic acid. The number of cells analyzed is indicated in the upper left corner of each graph. The arrows indicate the mean circularity value determined for the population of cells, and the asterisks indicate values that are significantly different from the untreated control at ; ; and .

(a)

(b) Untreated control

(c) 0.3 μM isoproterenol

(d) 100 μM oleic acid

(e) 200 μM oleic acid

(f) 0.3 μM isoproterenol + 100 μM oleic acid

(g) 0.3 μM isoproterenol + 200 μM oleic acid