Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth

Figure 1

hESCs were grown on irradiated feeder cells, Matrigel, or methanol fixed xeno-free ECM. (a) hESCs were grown on irradiated MEF feeder cells in KSR complete medium (10x); (b) hESCs were grown on fixed xeno-free ECM in TeSR-E8 (10x); (c) hESCs were grown on Matrigel in TeSR-E8 (20x); (d) hESCs were grown on fixed xeno-free ECM in TeSR-E8 (20x).

(a)

(b)

(c)

(d)