Pretreatment of Adipose Derived Stem Cells with Curcumin Facilitates Myocardial Recovery via Antiapoptosis and Angiogenesis

Figure 5

Administration of pretreated ADSCs increases cells retention and decreases myocardial fibrosis and apoptosis after myocardial infarction at 7 days after transplantation. (a) Representative fluorescence microscopic images ADSCs (Dil⁺, red florescence), cardiomyocytes (cTnI, green florescence), and DAPI (blue fluorescence) at 1 week after injection. Quantitative analysis revealed that a higher retention of ADSCs in Cur-ADSCs group was observed. Scale bars = 50 μm. versus ADSCs. (b) Effect of ADSCs transplantation and/or pretreatment with curcumin on infarct size. Representative TTC staining images and quantitative analysis at 7 days after transplantation showed intramyocardial injection of ADSCs decreased the LV infarct size (). Scale bars = 100 μm. Statistical differences () are indicated from PBS () and ADSCs (#). (c) Measurement of apoptosis in the ischemic heart. Representative TUNEL staining images in the peri-infarct area of heart slices by immunofluorescence and quantitative analysis showed TUNEL positive cells were the least in the Cur-ADSCs group (). Scale bars = 100 μm. Statistical differences () are indicated from PBS () and ADSCs (#).

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