Modulation of Hyaluronan Synthesis by the Interaction between Mesenchymal Stem Cells and Osteoarthritic Chondrocytes

Figure 6

OA-chondrocytes produce higher IL-6 and IL-8 levels than BM-MSCs and cocultured cells and decrease these cytokines. Interleukin (IL) production for 3 and 6 days in monoculture (; BM-MSCs and chondrocytes) or in coculture (). IL-6 concentration (ng/mL/cell) (a). IL-8 concentration (ng/mL/cell) (b). Overall, bars represent the mean value with the SEM; bars with gray line (expected coculture) show expected ILs synthesis in coculture, based on production of monoculture and cell number counted. Table shows cell number in each group. Statistical significance based on a two-way ANOVA with Tukey’s posttest was set according to the number of asterisks, as follows: , , and . Tendency of statistical differences between cell culture groups was identified by #.

(a)

(b)