Bone Marrow-Derived Multipotent Stromal Cells Promote Myocardial Fibrosis and Reverse Remodeling of the Left Ventricle

Figure 2

Homing of MSCs following intracoronary infusion. Fluorescence and light microscopy of heart cryosections following the autologous transplantation. (a) The red fluorescent cells are observed only in the affected myocardium (the areas are designated S for scar, NM for normal myocardium, and PM for perifocal myocardium (border zone)). (b) The labeled cells are not found in the border myocardium in the vicinity of cardiac myocytes (connexin-43-positive, green). (c) Typical images subject to quantitative analysis: (i) 10 min after infusion the labeled autologous MSCs are attached to the inner surface of vascular wall; (ii) at p/t day 14 (ii) the autologous MSCs and (iii) the allogeneic MSCs are found in the scar only. The diagrams show numbers of the label-presenting cells in heart, spleen, and liver counted in 37070 μm² field of view (d) at p/t day 14 and (e) at p/t day 30. The data are presented as mean ± SEM with asterisks indicating significant differences. Scale bars: (a), 100 μm; (b) and (c)i, 25 μm; (c)ii and (c)iii, 50 μm.

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