Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Relieve Acute Myocardial Ischemic Injury

Figure 6

hucMSC-exosomes reduced cell apoptosis and promoted cell proliferation. The apoptotic cells in the ischemic zone of myocardial tissue sections (a). Scale bar = 50 μm. The numbers of TUNEL positive cells in 3 randomly selected fields at 200 times magnification for each rat (b). versus AMI + PBS. Immunohistochemical staining of Ki67 expression in myocardial tissue from the border zone of an infarcted left ventricle (c). The black arrows indicate Ki67 positive cells. Scale bar = 50 μm. The numbers of Ki67 positive cells in 3 randomly selected fields at 200 times magnification for each rat (d). versus AMI + PBS. The protein of Bax and Bcl-2 in the myocardial cells H9C2(2-1) cultured under hypoxic conditions was evaluated by western blot (e). Statistical analysis of the ratio of Bcl-2 to Bax (f). versus Hypoxia + PBS.

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