Reprogramming with Small Molecules instead of Exogenous Transcription Factors

Figure 2

Small molecules based iPSC generation. Small molecules can substitute for all TFs through epigenetic modifications and signaling pathway regulations. Hou et al. reported that CiPSC generation from mEF was carried out in 3 steps of 16–20 days in VC6TF treatment and then 12–20 days in VC6TFZ and followed by 2i compounds regulations for 1 week. The somatic cell, mouse embryonic fibroblast (mEF) cell, undergoes dedifferentiation and gains multipotent stem cell characteristics under the treatments of VC6TF and VC6TFZ steps and finally obtains the full pluripotency in the medium containing 2i compounds (Chir99021 and PD0325901).