Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro

Figure 4

Seven migration related factors induced by supernatant of hypoxia CFs culture were upregulated in mRNA transcription level. VEGF (a), TNF-α (b), MMP-9 (c), MMP-2 (d), SCF (e), TIMP-1 (f), EGF (g), SDF-1 (h), bFGF (i), G-CSF (j), HGF (k), HIF-1α (l), and MCP-1 (m) mRNA transcription levels in supernatants of CFs under different period of hypoxia. mRNA levels of VEGF, TNF-α, MMP-9, and MMP-2 mRNA peaked at 24 h, while mRNA levels of SCF, SDF-1, bFGF, G-CSF, HGF, HIF-1α, and MCP-1 declined in a timely dependent manner with the period of hypoxia treatment. Lastly, mRNA levels of EGF and TIMP-1 showed no change with hypoxia treatment at all. Results are shown as the mean ± SD values. Experiments were repeated three times. indicates compared to the 0 h group. indicates compared to the 0 h group. # indicates compared to the 24 h group. ## indicates compared to the 24 h group.

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