Mesenchymal Stem/Stromal Cells Derived from Induced Pluripotent Stem Cells Support CD34^pos Hematopoietic Stem Cell Propagation and Suppress Inflammatory Reaction

Figure 2

Derivation and enrichment of MSCs from human iPS cells. (a) Schematic stepwise protocol for differentiation and selection of MSC-like cells from human iPS cells. (b) phase-contrast photos demonstrating Epithelial-Mesenchymal transition in cellular morphology. (c) FACS dot blot showing intermediate (R1) and highly (P2) double-positive cells. Highly positive CD-73 and CD-105 (R2) were sorted for upcoming experiments. (d) iPS-MSCs after sorting showed more homogenous mesenchymal morphology expressing GFP/dTom.

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