Mesenchymal Stem/Stromal Cells Derived from Induced Pluripotent Stem Cells Support CD34^pos Hematopoietic Stem Cell Propagation and Suppress Inflammatory Reaction

Figure 5

Coculture of CD34⁺ PBMCs with human iPSC-MSCs. (a) Cell layers of iPSC-MSCs and BMSCs were established on 1% gelatin precoated 24-well plates (80% confluent). CD34⁺ PBSCs were applied onto the stromal layers. The cocultures were incubated for 20 days. Nonadherent viable cells were counted at the indicated time points, (b) human CD34⁺ were plated with hiPSC-MSCs in 0.5 mL of methylcellulose media containing human recombinant IL-3, SCF, and Epo. The plates were incubated for 20 days following which progenitors were scored, (c) surface markers expression on CD34⁺ cells after coculture with mesenchymal stromal cells. The results represent the mean (±SD) of three replicates. : significance difference with CD34 without stroma .

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