In Vitro Effects of Strontium on Proliferation and Osteoinduction of Human Preadipocytes

Figure 2

Observation in light microscopy of osteogenic differentiation of the PA20-h5 line at 0 days ((a), (d)), 14 days ((b), (e)), and 35 days of induction ((c), (f)). Cytochemical staining for ALP ((a), (b), and (c)): positive cells stained in red and nuclei counterstained in blue (observation in brightfield microscopy, 20x objective). Cytochemical staining for calcium mineralized deposits ((d), (e), and (f)): deposits stained in red-orange (observation in phase contrast microscopy, 20x objective).