In Vitro Effects of Strontium on Proliferation and Osteoinduction of Human Preadipocytes

Table 3

Primers and TaqMan probes used for qRT-PCR.

Gene	Primer sequences (5′-3′) and TaqMan probes	Amplicon size (bp)	(°C)
RPS18 FOR; RPS18 REV; probe	TCTTCCACAGGAGGCCTAC; GATGGCAAAGGCTATTTTCCG; F/TTCAGGGAT/ZEN/CACTAGAGACATGGCTGC/Q	132	60
ALP195 FOR; ALP195 REV; probe	CCCGTGGCAACTCTATCTTTG; CATACAGGATGGCAGTGAAGG; F/TTCTTGTCT/ZEN/GTGTCACTCAGCATGGG/Q	78	60
DKK1 FOR; DKK1 REV; probe	TGATCATAGCACCTTGGATGG; ACACAATCCTGAGGCACAG; F/CTGATGACC/ZEN/GGAGACAAACAGAACCTT/Q	121	60
RUNX2 FOR; RUNX2 REV; probe	AGGGACTATGGCATCAAACAG; CTTCACGTCGCTCATTTTGC; F/TCTTTTGGA/ZEN/TCCGAGCACCAGCC/Q	135	60

TaqMan probes with F as reporter fluorochrome (6-carboxyfluorescein [6-FAM]) and Q as quencher.
Fluorochrome (Iowa Black FQ); bp, base paris of amplicon size; , melting temperature (°C).