Occurring of In Vitro Functional Vasculogenic Pericytes from Human Circulating Early Endothelial Precursor Cell Culture

Figure 4

Vasculogenic potential of human Progenitor Perivascular Cells (hPPCs) assessment in vitro. (a)–(c) Capillary network formation in Matrigel (40x magnification) after 3 hours (a), 8 hours (b), and 7 days (c). (d) Assessment of vasculogenic potential in nonadherent cells with hematopoietic profile after 8 hours (40x magnification). (e)-(f) Immunofluorescence staining of cells involved in tubule-like structures recovered from Matrigel at 7 days: CD34-positive cells (green, (e)) and KDR-positive cells (green, (f)). Nuclei are counterstained with DAPI (blue). 400x magnification. (g)-(h) Assessment of vasculogenic potential in sorted cells: CD146+ (g) and CD146− cells (h) after 8 hours (40x magnification).

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