Low Oxygen Tension Enhances Osteogenic Potential of Bone Marrow-Derived Mesenchymal Stem Cells with Osteonecrosis-Related Functional Impairment

<div>Effect of low oxygen (2%) on MSC osteogenic differentiation. (a) The mineralization nodes were monitored by Alizarin Red S staining 14 days after cells were induced in an osteogenic induction medium. (b) Alkaline phosphatase staining of MSCs after osteogenic differentiation. (c) Immunostaining for OCN and COL I (Texas-Red, a1-h1) with nuclear counterstained (DAPI-blue, a2-h2) 2 weeks after osteogenic differentiation (×400). (a3-h3) Merge. (d) ALP activities in BMSCs. (e) OCN mRNA expression was examined by RT-PCR at day 14. (f) COL I mRNA expression was examined by RT-PCR at day 14. 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Stem Cells International

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Figure 4

Figure 4: Low Oxygen Tension Enhances Osteogenic Potential of Bone Marrow-Derived Mesenchymal Stem Cells with Osteonecrosis-Related Functional Impairment 

Figure 4 | Low Oxygen Tension Enhances Osteogenic Potential of Bone Marrow-Derived Mesenchymal Stem Cells with Osteonecrosis-Related Functional Impairment 