Periodontal Ligament Stem Cells: Current Status, Concerns, and Future Prospects

Figure 1

Optimized conditions for isolation and culture of periodontal ligament stem cells. This represents an improved protocol for isolation and culture of PDLSCs. Optimal culture methods and conditions such as using enzymatic digestion, α-MEM, and seeding cells at low density under hypoxia are recommended. Sequential use of different growth factors is effective for cell expansion and differentiation.