Dual Inhibition of Activin/Nodal/TGF-β and BMP Signaling Pathways by SB431542 and Dorsomorphin Induces Neuronal Differentiation of Human Adipose Derived Stem Cells

Figure 6

hADSCs can differentiate into Schwann cell phenotype. hADSCs were grown in BM and nerve induction medium (NIM) for 14 days. Using gene specific primers, mRNA expression of Schwann cell markers P0, p75NTR, GFAP, and S100 was determined from the cDNA templates. The PCR mixture was separated into a 1% agarose gel and the bands were visualized by ethidium bromide staining of the gels. The band intensities were quantified using ImageJ software.