Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ

Figure 1

Human ASCs were induced to differentiate into SMCs by treatment with TGF-β1 and BMP4 for 7 days. (a) Quantitative real-time PCR experiments were carried out to measure the expression level of smooth muscle specific markers. (b, c) Time course of the expression of SMC differentiation markers detected by qRT-PCR and western blot analysis, respectively. (d) Immunofluorescent staining of smooth muscle specific markers (red), respectively. Nuclear were counterstained with DAPI (blue). Scale bars: 100 μm. Data represent means ± SE, ( versus controls).

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