Cytokines TNF-α, IL-6, IL-17F, and IL-4 Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells

Figure 3

Comparative analysis of the effect of pro- and anti-inflammatory cytokines on osteogenic differentiation of hASCs. ASCs were stimulated for 72 h with proinflammatory cytokines TNF-α, IL-6, IL-8, and IL-17F and the anti-inflammatory cytokine IL-4 (10 ng/mL). (a) TNF-α and IL-6 increased ALP activity at 7 days and IL-17F at 48 h and day 7. IL-4 increased ALP activity at day 4. Results are mean SD, . effect of cytokine treatment, . (b) ASCs showed enhanced mineralization using alizarin red staining after IL-6 treatment at day 14 compared to untreated controls. TNF-α, IL-8, IL-17F, and IL-4 decreased mineralization of hASCs at day 14 compared to untreated controls.

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