Research Article
Slowly Delivered Icariin/Allogeneic Bone Marrow-Derived Mesenchymal Stem Cells to Promote the Healing of Calvarial Critical-Size Bone Defects
Figure 2
Time-course and dose-dependent tests to select the optimal concentration of icariin for inducing the osteogenic differentiation of BMSCs. Icariin of 0, 10−9, 10−8, 10−7, 10−6, 10−5, or 10−4 mol/L was used to treat preosteoblasts for 1 day, 3 days, 5 days, and 7 days. (a) Cell proliferation assays; (b) alkaline phosphatase (ALP) activity assays. All data are presented as mean values together with the standard deviation (SD). ; ; indicating the statistical difference between the indicated group and the control group (blank) at the same time point.
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