Slowly Delivered Icariin/Allogeneic Bone Marrow-Derived Mesenchymal Stem Cells to Promote the Healing of Calvarial Critical-Size Bone Defects

Table 1

Primer sequences for real-time quantitative polymerase chain reaction analysis of the expression of osteogenic genes (Runx2, alkaline phosphatase (ALP), and osteocalcin (OCN)) and angiogenic genes (vascular endothelial growth factor (VEGF) and fibroblast growth factors (FGF)).

Gene	Primers (F = forward; R = reverse)
ALP	F: 5′-GTC CCA CAA GAG CCC ACA AT-3′; R: 5′-CAA CGG CAG AGC CAG GAA T-3′
OCN	F: 5′-CAG TAA GGT GGT GAA TAG ACT CCG-3′; R: 5′-GGT GCC ATA GAT GCG CTT G-3′
Runx2	F: 5′-TCT TCC CAA AGC CAG AGC G-3′; R: 5′-TGC CAT TCG AGG TGG TCG-3′
VEGF	F: 5′-CTT GAG TTG GGA GGA GGA TG-3′; R: 5′-TGG CAG GCA AAC AGA CTT C-3′
FGF	F: 5′-CTC TGT CTC CCG CAC CCT AT-3′; R: 5′-CCT TCC ACC CAA AGC AGT AG-3′
GAPDH	F: 5′-GGC AAG TTC AAC GGC ACA GT-3′; R: 5′-GCC AGT AGA CTC CAC GAC AT-3′