Lovastatin Decreases the Expression of CD133 and Influences the Differentiation Potential of Human Embryonic Stem Cells

Figure 1

Lovastatin treatment differently affects the expression of pluripotency markers in hES cells. (a) Flow cytometric analysis of the expression of the pluripotency markers NANOG, OCT4, and SOX2 in hES cells treated with lovastatin (20 μM lovastatin for 48 h) and those untreated. Fixed and permeabilised cells were stained with anti-SSEA-3 (Alexa Fluor 488 conjugate), anti-NANOG (PE), anti-OCT4 (Alexa Fluor 647), and anti-SOX2 (PerCp Cy5.5 conjugate) antibodies and with DAPI. For analysis cellular debris and doublets were excluded. (b) The morphological changes in colony structure of lovastatin treated hES cells. (c) The number of cells expressing pluripotency markers. Results are shown as mean ± SD (). (d) The number of cells in one well after 48 h of treatment with lovastatin. (e) Changes in SSEA-3 expression in untreated and lovastatin treated (10–40 μM for 24 h) hES cells.

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