Stem Cells International / 2016 / Article / Tab 1 / Research Article
Mutation-Specific Phenotypes in hiPSC-Derived Cardiomyocytes Carrying Either Myosin-Binding Protein C Or α -Tropomyosin Mutation for Hypertrophic Cardiomyopathy Table 1 TaqMan assays used in qRT-PCR experiments.
Gene Description/alias Function TaqMan assay ID EEF1A1 Eukaryotic translation elongation factor 1 alpha 1 Housekeeping gene Hs00265885_g1 GAPDH Glyceraldehyde-3-phosphate dehydrogenase Housekeeping gene Hs02758991_g1 TNNT2 Troponin T Sarcomeric gene Hs00165960_m1 MYH6 Myosin heavy chain 6 Sarcomeric gene Hs01101425_m1 ACTN2 α -actinin 2Sarcomeric gene Hs00153809_m1 TPM1 α -tropomyosinSarcomeric gene Hs00165966_m1 MYBPC3 Myosin-binding protein C Sarcomeric gene Hs00165232_m1 TTN Titin Sarcomeric gene Hs00399225_m1 TNNC1 Troponin C type 1 Sarcomeric gene Hs00896999_g1 MYL9 Myosin, light chain 2/MLC2 Sarcomeric gene Hs00697086_m1 MYL7 Myosin, light chain 7 Sarcomeric gene Hs01085598_g1 NPPA Natriuretic peptide A Hypertrophy marker Hs01081097_m1 NPPB Natriuretic peptide B Hypertrophy marker Hs01057466_g1 HCN4 Hyperpolarization activated cyclic nucleotide-gated potassium channel 4 Potassium channel Hs00975492_m1 KCNQ1 Voltage-gated potassium channel, KQT-like subfamily, member 1 Potassium channel Hs00923522_m1 CACNA1C Voltage-dependent calcium channel, L type, alpha 1C subunit/CaCNA1.2 Calcium channel Hs00167681_m1 SCN5A Voltage-gated sodium channel, V type, alpha subunit Sodium channel Hs00165693_m1 SLC8A1 Solute carrier family 8, member 1/NCX1 Na+ /Ca2+ exchanger Hs01062258_m1 PLN Phospholamban/PLB Protein kinase substrate Hs01848144_s1 ATP2A2 ATPase, Ca2+ transporting, cardiac muscle, slow twitch 2/SERCA2a Ca2+ -ATPase Hs00544877_m1 CASQ2 Calsequestrin Ca2+ binding protein in SR Hs00154286_m1 ITPR2 Inositol 1,4,5-trisphosphate receptor, type 2/IP3R2 Ca2+ receptor Hs00181916_m1 RYR2 Ryanodine receptor 2 (cardiac) Ryanodine receptor Hs00892883_m1
