Dynamic Tracking Human Mesenchymal Stem Cells Tropism following Smoke Inhalation Injury in NOD/SCID Mice

Figure 2

Characterization of human bone marrow-derived stem cells coexpressing luciferase and green fluorescent protein reporter genes (Luc-GFP-hBMSCs). (a) Flow cytometric analysis of Luc-GFP-hBMSCs. Luc-GFP-hBMSCs were harvested with 0.25% trypsin-EDTA and resuspended in phosphate-buffered saline supplemented with 2% fetal bovine serum. Following this, the expression levels of CD90, CD105, CD29, CD73, CD44, CD34, CD45, CD11c, and HLA-DR were measured by fluorescence-activated cell sorting analysis. (b)–(d) Luc-GFP-BMSCs were able to differentiate into adipocytes, osteoblasts, and chondrocytes in vitro as shown by positive Oil Red O staining (b), Alizarin red staining (c), and Alcian Blue staining (d). The green arrows indicate lipid droplets stained with Oil Red O, and the white arrows indicate calcium nodules stained with Alizarin red. Acid mucopolysaccharide of chondrocytes could be stained by Alcian Blue.

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