Dynamic Tracking Human Mesenchymal Stem Cells Tropism following Smoke Inhalation Injury in NOD/SCID Mice

Figure 5

Detection of human bone marrow-derived stem cells coexpressing luciferase and green fluorescent protein reporter genes in mouse tissues at 14 days after injection. (a) Expression of the firefly luciferase gene was detected by PCR. β-actin was used as a loading control. Panel 1: DNA marker. Panels 2–7: from left to right, human bone marrow-derived stem cells coexpressing luciferase and green fluorescent protein reporter genes (Luc-GFP-hBMSCs), liver from a control mouse treated with Luc-GFP-hBMSCs, liver from a mouse with smoke inhalation injury (SII) treated with Luc-GFP-hBMSCs, lung from a control mouse treated with Luc-GFP-hBMSCs, and lung from a mouse with SII treated with Luc-GFP-hBMSCs. Panels 8–12: corresponding β-actin levels from panels 2–6. Representative images from at least three independent experiments. (b) Luc-GFP-hBMSCs were detected by immunohistochemistry (anti-GFP) in the lung at day 14 after injection into mice with SII ().

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