Effects on Proliferation and Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells Engineered to Express Neurotrophic Factors

Figure 7

Effects of overexpression of NTFs from HUMSCs on the neurite outgrowth of embryonic chick DRG. (a) Representative images of E9 DRG culture for 24 hours (scale bar = 200 μm). (b) DRG cultured in blank culture medium extended short neurites. NT-3 (3.5 ng/mL), NGF (10 ng/mL), and overexpression of NTFs from HUMSCs treatment effectively promoted neurite outgrowth. Neurite extension of DRG treated with cell culture supernatant of HUMSCs (control) was similar to that of blank group. versus blank or control ().

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