Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels

Figure 6

Development of BCP60/40-based composites and BCP20/80-based composites. Human adipose stem cells were seeded on BCP60/40 or BCP20/80 and incorporated in fibrin gels. BCP20/80-based composites showed enhanced osteogenic differentiation and vasculogenic potential compared to BCP60/40-based composites in vitro. =, similar in both composites; <, higher in BCP20/80-based composites compared to BCP60/40-based composites; HA, hydroxyapatite; β-TCP, β-tricalcium phosphate; KI67, proliferation marker; RUNX2, runt-related transcription factor-2; ON, osteonectin; DMP1, dentin matrix acidic phosphoprotein-1; COL1, collagen-1; COL3, collagen-3; CD31, cluster of differentiation-31; VEGF165, vascular endothelial growth factor-165; VEGF189, vascular endothelial growth factor-189; EDN1, endothelin-1.