Research Article
High Content Analysis of Human Pluripotent Stem Cell Derived Hepatocytes Reveals Drug Induced Steatosis and Phospholipidosis
Table 5
Comparison of assay imprecision for each parameter measured in hiPSC derived hepatocytes (hiPS-HEP) and HepG2.
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Imprecisions of cell-to-cell within a well, well-to-well within a plate, plate-to-plate within a batch, and batch-to-batch are compared for all parameters measured. For calculating well-to-well variance, a mean, SD, and CV% were calculated for every control well () and an average CV% of all the eight plates was reported for well-to-well variation. The well-to-well mean values for each parameter within a batch () were then averaged and its CV% was calculated. The average CV% from the four batches was used to show plate-to-plate variation. For batch-to-batch variance, the mean values from each plate were averaged and its CV% was reported (). |