The New Role of CD163 in the Differentiation of Bone Marrow Stromal Cells into Vascular Endothelial-Like Cells

Figure 3

The protein level of CD163 influenced ABO-induced BMSC differentiation. For CD163 interference, cells were subjected with 80 nmol/L scrambled siRNA or 20, 40, and 80 nmol/L CD163 siRNA for 6 h and cultured for 48 h. Scr: scrambled RNA as negative control. (a) Western blot analysis of CD163 RNA interference efficiency. (b) Relative levels of CD163 protein were calculated based on densitometry analysis. (c) Western blot analysis of the protein level of CD31, Flk-1, Hmbox1, and CD163 after overexpression or knockdown of CD163 with or without 50 μM ABO treatment (DMSO as control). ((d)-(e)) Relative protein levels of CD31, Flk-1, Hmbox1, and CD163 were calculated based on densitometry analysis. Data are mean ± SEM. versus ABO group, versus control (Ctr), .

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