Extracellular Recordings of Patterned Human Pluripotent Stem Cell-Derived Cardiomyocytes on Aligned Fibers

<div>IMR hiPS-cardiomyocytes (CMs) cultured on aligned fiber (AF)-coated and gelatin-coated (Flat) microelectrode arrays (MEA). (a) Phase contrast images of CMs plated on the MEA for 2 days: the scale bar in = 200 <i>μ</i>m. (b, c) Fluorescence images of CMs cultured for 14 days on MEA. (b) MLC2v is shown in red, (c) <i>β</i>-MHC in green, and DAPI in blue. The scale bar = 200 <i>μ</i>m. The color on fiber is due to autofluorescence of PMGI. White arrow marked the fiber orientation.</div>

Stem Cells International

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Figure 3

Figure 3: Extracellular Recordings of Patterned Human Pluripotent Stem Cell-Derived Cardiomyocytes on Aligned Fibers 