The Novel Methods for Analysis of Exosomes Released from Endothelial Cells and Endothelial Progenitor Cells

Figure 3

The efficiencies and specificities of the methods by combining microbeads with NTA for purifying and detecting EPC-EXs. (a) The purification efficiency and specificity of EPC-EXs in the total EPC-EXs, which were collected from EPC culture medium by ultracentrifuge and isolated by various microbeads-conjugated antibodies against EPC specific markers (CD34, as well as negative controls, CD105, CD41, and CD235a). (b) The detection efficiency and specificity of EPC-EXs in the total CD34⁺ EXs that were labeled with CD144-, or KDR-, or Annexin V-, or CD63-conjugated Q-dots upon detection by fluorescence NTA. (c) The overall efficiency for measuring the CD34⁺ EXs colabeled with CD144-, or KDR-, or Annexin V-, or CD63-conjugated Q-dots in the total EC-EXs. (d) Representative plots showing the size/concentration distribution of the CD34⁺ beads isolated EXs under fluorescence/nonfluorescence modes. Black curve: CD34⁺ EXs measured under light scatter (nonfluorescence) mode. Yellow curve: CD34⁺Q-dots⁺ EXs measured under fluorescence mode. Black dash line: 200 nm landmark. /group.

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