The Novel Methods for Analysis of Exosomes Released from Endothelial Cells and Endothelial Progenitor Cells

Figure 5

High recovery and detection efficiency of EPC-EXs from particle-free plasma by using microbead purification and fluorescence NTA detection methods. (a) EPC-EXs were recovered from particle-free plasma by using microbeads conjugated with various antibodies and analyzed by NTA. (b) The detection efficiency of recovered CD34⁺ EXs that were labeled with secondary antibodies (CD144, or KDR, or Annexin V, or CD63) conjugated with Q-dots and analyzed by fluorescent NTA. (c) The overall efficiency for measuring the recovered CD34⁺ EXs colabeled with CD144-, or KDR-, or Annexin V-, or CD63-conjugated Q-dots. (d) The absolute number of recovered CD34⁺ EXs that were positive for CD144, or KDR, or Annexin V, or CD63 per mL particle-free plasma. /group.

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