Research Article
Endothelium-Independent Hypoxic Contraction Is Prevented Specifically by Nitroglycerin via Inhibition of Akt Kinase in Porcine Coronary Artery
Figure 2
Effects of NTG and DETA NONOate on protein levels of Akt-p (S473) and total Akt. Akt-p (S473) was significantly decreased after NTG (10−5 M) treatment for 15 min (a) or DETA NONOate (NO, 10−4 M) treatment for 1 min (c), which could be recovered by ODQ (3 × 10−5 M; (b) and (d)). The upper panels are western blots. The lower panels are the summaries of densitometric scanning of proteins expressed as ratio of Akt-p (S473) to total Akt. Data shown as means ± SEM; –8 for each group. different from the control group (); different from the control group (, (a) and (c)); different from the NO group (, (d)).
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