Research Article
Hepatocyte Growth Factor Effects on Mesenchymal Stem Cells Derived from Human Arteries: A Novel Strategy to Accelerate Vascular Ulcer Wound Healing
Figure 1
hVW-MSC aspect and HGF/c-MET expression. (a) hVW-MSCs were enzymatically isolated from human arteries and, at passage 3, they exhibited adherence to the plastic substrate and a typical spindle-shaped morphology. Scale bars = 100 μm. (b) Flow cytometry analysis of mesenchymal, stemness, pericyte, and hematopoietic and vascular markers expressed in our cell model of hVW-MSCs. Values are reported in percentage of positivity. (c) Basal expression of HGF (252 bp) and c-MET (440 bp) mRNA in hVW-MSCs, detected by RT-PCR. (d) c-Met and HGF protein detection on cell lysates of hVW-MSCs exposed to HGF/SF (0, 2.5, 5, and 10 ng/mL) for 24 hrs.
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