XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4/Sox2/c-Myc

Figure 2

Establishment of XPC overexpression DPCs model. Expression of pCDH-CMV-XPC-EF1-copGFP plasmid in HEK293T cells and DPCs at P2 (a1–a6). Strong green fluorescence was detected in HEK293T cells (a2, ×50) and DPCs (a5, ×100) after transfection. Combined figures of the bright field and fluorescence figure (a3, a6). Western blot and real-time PCR showed that the expression of XPC was enhanced in XPC+/DPCs at P2 at both protein and mRNA level compared with vector group, and XPC maintained high expression level in DPCs at P3 and P7 () (b1, b2).