Effects of Intermittent Administration of Parathyroid Hormone (1-34) on Bone Differentiation in Stromal Precursor Antigen-1 Positive Human Periodontal Ligament Stem Cells

Figure 2

STRO-1(+) hPDLSCs exhibited strong osteogenic capacity. (a) Immunostaining for Stro-1 in isolated STRO-1(+) and STRO-1(−) hPDLSCs (magnification: 50x). The surface marker Stro-1 was stained with FITC and visualized using fluorescence microscopy. Nuclei were stained with DAPI (blue). (b) ALP activity in STRO-1(+) and STRO-1(−) hPDLSCs. (c) Osteogenic differentiated cells were stained with Alizarin red (magnification: 100x). (d) Real-time PCR and western blot analyses of Runx2 expression in STRO-1(+) and STRO-1(−) hPDLSCs. The expression of each target was normalized to that of GAPDH. Data are presented as the means ± SDs of three independent experiments performed in duplicate. , .

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