Effects of Intermittent Administration of Parathyroid Hormone (1-34) on Bone Differentiation in Stromal Precursor Antigen-1 Positive Human Periodontal Ligament Stem Cells

Figure 4

The osteogenic capacity of STRO-1(+) hPDLSCs was enhanced by PTH. (a) Real-time PCR and western blot analyses of PTH1R, RUNX2, and SP7 expression in STRO-1(+) hPDLSCs before and after PTH treatment and induction by osteogenic-induction medium (OM). The expression of each target was normalized to that of GAPDH. (b) ALP activity in STRO-1(+) hPDLSCs. (c) Cells subjected to osteogenic differentiation were stained with Alizarin red (magnification: 100x). Data are presented as the means ± SDs of three independent experiments performed in duplicate. , .

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