Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells

Figure 2

In the lack of Rybp there is no change in silencing of key pluripotency markers (Oct4, Nanog) during in vitro neural differentiation. Relative gene expressions of Rybp (a), Oct4 (b), Nanog (c), and Sox2 (d) were analyzed by qRT-PCR. For the analysis RNA was extracted and reverse-transcribed from differentiated neural cell culture generated from and ESCs. Samples are collected at days 0, 3, 7, 10, and 14 of differentiation. The expression of the indicated markers was normalized to Hprt expression, which was used as an internal control. Means are standard deviation. Values of were accepted as significant (; ; ). Statistical method: test type 3.

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