Direct Reprogramming of Mouse Fibroblasts to Neural Stem Cells by Small Molecules

Figure 2

Differentiation of SMINS-TTF-3 cells in vitro. (a-b) SMINS-TTF-3 cells spontaneously differentiated into astrocytes marked by GFAP (green) and neurons marked by Tuj1 (red) in spontaneous medium for one week. (c-d) SMINS-TTF-3 cells spontaneously differentiate into neurons marked by Map2 (green) and oligodendrocytes marked by O4 (red) in spontaneous medium for three weeks. DAPI was used for nuclei counterstaining (blue). Scale bar: 100 μm (a and b) and 10 μm (c and d).

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