Aryl Hydrocarbon Receptor Deficiency in an Exon 3 Deletion Mouse Model Promotes Hematopoietic Stem Cell Proliferation and Impacts Endosteal Niche Cells

Figure 3

AHR-deficient BM cells have changes in long term competitive transplantation ability in serial transplantation. Bone marrow cells from donor AHR-KO (CD45.2⁺) or WT (CD45.2⁺) and recipient competitor CD45.1⁺ mice were mixed together at a ratio of 1 : 1 (1 × 10⁶ cells each) and injected into irradiated CD45.1⁺ recipient mice (8 each for donor AHR-KO and WT) for primary BM transplantation. Bone marrow cells were isolated from primary recipients after 16 weeks and serially transplanted in recipient mice as described in Section 2. (a) Bone marrow and (b) spleen cells were analyzed for CD45.2⁺ (donor) and CD45.1⁺ (recipient) origin after 16 weeks at each stage of transplantation. An analysis of BM cells was done for primitive hematopoietic and progenitor cells: (c) primary, (d) secondary bone, and (e) tertiary transplantation (LTHSC = LSK, CD135⁻, CD48⁻, CD150⁺; STHSC = LSK CD135⁻ CD48⁻ CD150⁻; MPP1 = LSK CD135⁻ CD48⁺ CD150⁻; MPP2 = LSK CD135⁻ CD48⁺ CD150⁺). Data shown are the mean ± SD. significantly different from WT control () ().

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