Human Umbilical Cord Blood-Derived Serum for Culturing the Supportive Feeder Cells of Human Pluripotent Stem Cell Lines

Figure 2

Effect of serum supplementation on cell proliferation and karyotype stability of human foreskin fibroblasts. The cell proliferation of human foreskin fibroblasts (HFFs) was evaluated by determination of their population doubling time (PDT). The ability of HFFs to maintain their normal karyotype was assessed by the G-banding method. The PDT of HFFs cultured in the media containing human cord blood-derived serum (hUCS; HFF-hUCS) and fetal bovine serum (FBS; HFF-FBS) from p4 + 1 to p4 + 13 were determined. The HFF-hUCS from p4 + 3 to p4 + 13 displayed a significantly shorter () PDT than HFF-FBS (a). The karyotype analysis of HFF-FBS and HFF-hUCS demonstrated that both HFFs cultured in hUCS- or FBS-containing media maintained a normal 46,XY karyotype after prolonged culture (b). Error bars represent the standard error of mean (SEM). HFF = human foreskin fibroblasts, FBS = fetal bovine serum, hUCS = human cord blood-derived serum, and NS = not significant. Scale bars = 200 μm, .

(a)

(b)