Human Umbilical Cord Blood-Derived Serum for Culturing the Supportive Feeder Cells of Human Pluripotent Stem Cell Lines

Figure 3

Morphology and gene expression of inactivated human foreskin fibroblasts prior to use as the feeder layer. HFF-hUCS and HFF-FBS survived inactivation through mitomycin-C treatment and maintained typical fibroblast-like features (a). RT-PCR was performed for the detection of supportive feeder cell-related genes. Inactivated HFF-hUCS and inactivated HFF-FBS expressed Activin A, FGF2, TGF-β1, and BMP-4. GAPDH was used as the house keeping gene (b). BMP = bone morphogenetic protein, FGF = fibroblast growth factors, TGF = transforming growth factor, HFF = human foreskin fibroblasts, F and FBS = fetal bovine serum, hU and hUCS = human cord blood-derived serum, RT-PCR = reverse transcription polymerase chain reaction, and GAPDH = glyceraldehyde 3-phosphate dehydrogenase. Scale bars = 200 μm.

(a)

(b)