Tissue-Related Hypoxia Attenuates Proinflammatory Effects of Allogeneic PBMCs on Adipose-Derived Stromal Cells In Vitro

Figure 4

ASC functions after paracrine interaction with PHA-stimulated PBMCs. (a) ASC viability: percentage of viable, apoptotic, and necrotic cells in monoculture and after coculture during 72 h at 20% and 5% O₂. (b) ASC proliferation: the change in cell number in monoculture and after coculture during 72 h at 20% and 5% O₂. (c) Matrix mineralization of ASCs at 20% (1) and 5% O₂ (2), alizarin red staining: bar 200 μm. (d) Osteodifferentiation: matrix mineralization in monocultured and cocultured ASCs. Significant difference between ASC under 20% and 5% O₂ in monoculture. Significant difference between ASC under 20% and 5% O₂ in coculture.

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