Factor-Reduced Human Induced Pluripotent Stem Cells Efficiently Differentiate into Neurons Independent of the Number of Reprogramming Factors

Figure 1

Comparison of neuroectodermal differentiation potential of human factor-reduced iPSCs. (a) Representative images of TUJ1⁺, GFAP⁺, GALC⁺, MAP2⁺, TH⁺, and Synaptophysin⁺ colonies generated by iPSCs derived from human fetal NSCs by one-factor reprogramming (OCT4, ), by iPSCs derived from human fetal NSCs by two-factor reprogramming (OCT4, KLF4; ), by iPSCs derived from human fibroblasts by three-factor reprogramming (OCT4, KLF4, and SOX2; ), and by iPSCs derived from human fibroblasts by classical four-factor reprogramming (OCT4, KLF4, SOX2, and c-Myc; ) after 3-4 weeks of differentiation on PA6 stromal cells. Scale bars represent 200 μm. (b) Values are means ± SEM from at least three to four independent experiments. , post hoc t-tests with Bonferroni adjustment in comparison to .

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