Theoretical and Practical Issues That Are Relevant When Scaling Up hMSC Microcarrier Production Processes

Figure 1

Results of hASC cultivations in the Corning spinner flasks. (a) Time-dependent profiles of living cell densities. The dotted lines represent the simulated growth characteristics of the hASCs in the exponential growth phase, based on the calculated specific growth rates. The black arrow indicates the 50% medium exchange on day 4. (b) Comparison of maximum expansion factors calculated for day 7. A one-way ANOVA (Holm-Sidak method, ; ) with pairwise comparison was performed for the maximum EFs. ns = not significant. The error bars represent the standard deviations of the EF given by the two cases of independent spinner cultivation per condition.

(a)

(b)